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Research and Education
Plasmid DNA Amplification and Isolation | Day 3: Plasmid DNA Isolation
Protocol: Bacterial Culturing from Stabs and Plasmid DNA Isolation Protocol Prepared by: Michael Sandway, Haoyu Xu This three-day protocol is intended for use with bacterial stabs already containing the plasmid, rather than purified plasmid DNA. Day One: 1. Autoclave 100 mL LB broth in an autoclave-safe glass bottle and let cool 2. Sterilize ...
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